MAGEA3 Monoclonal antibody
MAGEA3 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A9F3
Cat no : 60054-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue |
Positive IHC detected in | human lung cancer tissue, human oesophagus cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
60054-1-Ig targets MAGEA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MAGEA3 fusion protein Ag0265 相同性解析による交差性が予測される生物種 |
Full Name | melanoma antigen family A, 3 |
Calculated molecular weight | 314 aa, 35 kDa |
Observed molecular weight | 45-48 kDa |
GenBank accession number | BC000340 |
Gene symbol | MAGEA3 |
Gene ID (NCBI) | 4102 |
RRID | AB_2138124 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAGEA3 is a member of the MAGEA family, , which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.
Protocols
Product Specific Protocols | |
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WB protocol for MAGEA3 antibody 60054-1-Ig | Download protocol |
IHC protocol for MAGEA3 antibody 60054-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
PLoS One MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. | ||
J Transl Med Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk |