Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse brain using 60054-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 60054-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 60054-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 60054-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 60054-1-Ig
human testis tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 60054-1-Ig
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 10x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 40x lens).
IHC staining of human testis using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 60054-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

MAGEA3 Monoclonal antibody

MAGEA3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60054-1-Ig

Clone No.1A9F3

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue
Positive IHC detected in	human lung cancer tissue, human oesophagus cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

60054-1-Ig targets MAGEA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	MAGEA3 fusion protein Ag0265 相同性解析による交差性が予測される生物種
Full Name	melanoma antigen family A, 3
Calculated molecular weight	314 aa, 35 kDa
Observed molecular weight	45-48 kDa
GenBank accession number	BC000340
Gene Symbol	MAGEA3
Gene ID (NCBI)	4102
RRID	AB_2138124
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P43357
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAGEA3 is a member of the MAGEA family, , which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

Protocols

Product Specific Protocols
WB protocol for MAGEA3 antibody 60054-1-Ig	Download protocol
IHC protocol for MAGEA3 antibody 60054-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	PLoS One MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. Authors - Aaron Chen View Article
human	WB	J Transl Med Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk Authors - Hongquan Qin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 60054-1-Ig

WB analysis of rat brain using 60054-1-Ig

WB analysis of rat brain using 60054-1-Ig

WB analysis of mouse brain using 60054-1-Ig

WB analysis of human testis using 60054-1-Ig

WB analysis of fetal human brain using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human oesophagus cancer using 60054-1-Ig

IHC staining of human oesophagus cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human testis using 60054-1-Ig

IHC staining of human testis using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

IHC staining of human lung cancer using 60054-1-Ig

MAGEA3 Monoclonal antibody

MAGEA3 Monoclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation.

Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk