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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA3 Recombinant antibody, PBS Only (Capture/Detector)

MAGEA3 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240435F8

Cat no : 83480-3-PBS

Synonyms

HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00467-4

    Sandwich ELISA standard curve of MP00467-4

    Sandwich ELISA standard curve of MP00467-4, Human MAGEA3 Recombinant Matched Antibody Pair - PBS only. 83480-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0265. 83480-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP00467-4

    Sample test of MP00467-4

    The mean human MAGEA3 concentration was determined to be 2,719.3 pg/mL in BxPC-3 cell extract based on a 5.4 mg/mL extract load.

  • Sample test of MP00467-4

    Sample test of MP00467-4

    Serum of fourteen individual healthy human donors was measured. The human MAGEA3 concentration of detected samples was determined to be 1,950.7 pg/mL with a range of ND - 12,398.4 pg/mL.

  • Cytometric bead array standard curve of MP00467-1

    Cytometric bead array standard curve of MP00467-1

    Cytometric bead array standard curve of MP00467-1, MAGEA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83480-3-PBS. Detection antibody: 83480-2-PBS. Standard: Ag0265. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00467-2

    Cytometric bead array standard curve of MP00467-2

    Cytometric bead array standard curve of MP00467-2, MAGEA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83480-3-PBS. Detection antibody: 83480-4-PBS. Standard: Ag0265. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83480-3-PBS targets MAGEA3 as part of a matched antibody pair:

MP00467-1: 83480-3-PBS capture and 83480-2-PBS detection (validated in Cytometric bead array)

MP00467-2: 83480-3-PBS capture and 83480-4-PBS detection (validated in Cytometric bead array)

MP00467-4: 83480-6-PBS capture and 83480-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGEA3 fusion protein Ag0265 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 3
Calculated molecular weight 314 aa, 35 kDa
GenBank accession numberBC000340
Gene symbol MAGEA3
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.