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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA3 Recombinant antibody, PBS Only (Detector)

MAGEA3 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240435G9

Cat no : 83480-5-PBS

Synonyms

HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA3 Recombinant antibody (83480-5-RR)

    WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAGEA3 Recombinant antibody (83480-5-RR)

    WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MAGEA3 Recombinant antibody (83480-5-RR)

    IF Staining of A549 using 83480-5-RR (same clone as 83480-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MAGEA3 Recombinant antibody (83480-5-RR)

    FC experiment of HeLa using 83480-5-RR (same clone as 83480-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using MAGEA3 Recombinant antibody (83480-5-RR)

    FC experiment of Jurkat using 83480-5-RR (same clone as 83480-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00467-3

    Cytometric bead array standard curve of MP00467-3

    Cytometric bead array standard curve of MP00467-3, MAGEA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83480-1-PBS. Detection antibody: 83480-5-PBS. Standard: Ag0265. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83480-5-RR

    Affinity and Kinetic Characterization of 83480-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83480-5-PBS targets MAGEA3 as part of a matched antibody pair:

MP00467-3: 83480-1-PBS capture and 83480-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGEA3 fusion protein Ag0265 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 3
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight45 kDa
GenBank accession numberBC000340
Gene symbol MAGEA3
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.