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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12508-1-AP

Synonyms

MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA4 Polyclonal antibody (12508-1-AP)

    WB analysis using 12508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA4 Polyclonal antibody (12508-1-AP)

    IHC staining of human breast cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

  • Flow cytometry (FC) experiment of A431 cells using MAGEA4 Polyclonal antibody (12508-1-AP)

    FC experiment of A431 using 12508-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug MAGEA4 Polyclonal antibody (12508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inA431 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA4 fusion protein Ag3193 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 4
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017723
Gene symbol MAGEA4
Gene ID (NCBI) 4103
RRIDAB_2877863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGEA4, also named as MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

Protocols

Product Specific Protocols
WB protocol for MAGEA4 antibody 12508-1-APDownload protocol
IHC protocol for MAGEA4 antibody 12508-1-APDownload protocol
FC protocol for MAGEA4 antibody 12508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Endocrinol (Lausanne)

Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment

Authors - Yunpeng Wang
View Article