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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55109-1-AP

Synonyms

Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE X2, MAGE X2 antigen, MAGE4, MAGE4A, MAGE4B, MAGEA4, melanoma antigen family A, 4, Melanoma associated antigen 4

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using MAGEA4 Polyclonal antibody (55109-1-AP)

    WB analysis of human placenta using 55109-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55109-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MAGEA4 Polyclonal antibody (55109-1-AP)

    WB analysis of A375 using 55109-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 55109-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA4 Polyclonal antibody (55109-1-AP)

    IHC staining of human breast cancer using 55109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55109-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA4 Polyclonal antibody (55109-1-AP)

    IHC staining of human breast cancer using 55109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55109-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman placenta tissue, A375 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55109-1-AP targets MAGEA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 4
Calculated molecular weight 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberNM_001011548
Gene symbol MAGEA4
Gene ID (NCBI) 4103
RRIDAB_10858794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGEA4, also named as MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

Protocols

Product Specific Protocols
WB protocol for MAGEA4 antibody 55109-1-APDownload protocol
IHC protocol for MAGEA4 antibody 55109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols