• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

MAGEA6 Polyclonal antibody

MAGEA6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14602-1-AP

Synonyms

Melanoma-associated antigen 6, MAGE-6 antigen, MAGE6, MAGE3B antigen, CT1.6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MAGEA6 Polyclonal antibody (14602-1-AP)

    WB analysis using 14602-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAGEA6 Polyclonal antibody (14602-1-AP)

    WB analysis using 14602-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human lung cancer using 14602-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14602-1-AP (MAGEA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human lung cancer using 14602-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14602-1-AP (MAGEA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human breast cancer using 14602-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human breast cancer using 14602-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA6 Polyclonal antibody (14602-1-AP)

    IF Staining of A375 using 14602-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using MAGEA6 antibody (14602-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse testis tissue, A375 cells, rat testis tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14602-1-AP targets MAGEA6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA6 fusion protein Ag6148 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 6
Calculated molecular weight 35 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC067731
Gene symbol MAGEA6
Gene ID (NCBI) 4105
RRIDAB_2250093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA6 antibody 14602-1-APDownload protocol
IHC protocol for MAGEA6 antibody 14602-1-APDownload protocol
IF protocol for MAGEA6 antibody 14602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Neurol

Helios modulates the maturation of a CA1 neuronal subpopulation required for spatial memory formation.

Authors - Albert Giralt
View Article
humanIHC

Anticancer Res

Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer.

Authors - Miyo Endo
View Article
humanWB

Exp Ther Med

MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells.

Authors - Manyu Liu
  • KD Validated
View Article
humanIHC

Exp Ther Med

Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues.

Authors - Jun Hao
View Article