MAGEB18 Polyclonal antibody

MAGEB18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17494-1-AP

Synonyms

MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18


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Tested Applications

Positive WB detected inA375 cells, HeLa cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17494-1-AP targets MAGEB18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEB18 fusion protein Ag11624 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family B, 18
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC029525
Gene symbol MAGEB18
Gene ID (NCBI) 286514
RRIDAB_2138404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEB18 antibody 17494-1-APDownload protocol
IHC protocol for MAGEB18 antibody 17494-1-APDownload protocol
IF protocol for MAGEB18 antibody 17494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols