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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEB3 Polyclonal antibody, PBS Only

MAGEB3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18000-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEB3 Polyclonal antibody (18000-1-AP)

    WB analysis using 18000-1-AP (same clone as 18000-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18000-1-AP (MAGEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18000-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MAGEB3 Polyclonal antibody (18000-1-AP)

    IHC staining of mouse testis using 18000-1-AP (same clone as 18000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18000-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MAGEB3 Polyclonal antibody (18000-1-AP)

    IHC staining of mouse testis using 18000-1-AP (same clone as 18000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18000-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18000-1-PBS targets MAGEB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEB3 fusion protein Ag12476 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family B, 3
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC074756
Gene symbol MAGEB3
Gene ID (NCBI) 4114
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. MAGEB3, also named CT3.5, is a member of the MAGEB gene family.