MAGEC3 Polyclonal antibody
MAGEC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 21491-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, mouse thymus tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A375 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IP | See 1 publications below |
Product Information
21491-1-AP targets MAGEC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MAGEC3 fusion protein Ag15819 相同性解析による交差性が予測される生物種 |
Full Name | melanoma antigen family C, 3 |
Calculated molecular weight | 643 aa, 72 kDa |
Observed molecular weight | 70-72 kDa |
GenBank accession number | BC103893 |
Gene symbol | MAGEC3 |
Gene ID (NCBI) | 139081 |
RRID | AB_10734430 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
IHC protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
IF protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma. | ||
BMC Cancer Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells. |