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MAGED2 Polyclonal antibody
MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15252-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells, A431 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
15252-1-AP targets MAGED2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MAGED2 fusion protein Ag6828 相同性解析による交差性が予測される生物種 |
Full Name | melanoma antigen family D, 2 |
Calculated molecular weight | 65 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC000304 |
Gene symbol | MAGED2 |
Gene ID (NCBI) | 10916 |
RRID | AB_2138557 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for MAGED2 antibody 15252-1-AP | Download protocol |
IHC protocol for MAGED2 antibody 15252-1-AP | Download protocol |
IF protocol for MAGED2 antibody 15252-1-AP | Download protocol |
IP protocol for MAGED2 antibody 15252-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nephrol Dial Transplant MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells. | ||
J Neurosci MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation. | ||
Biochem Pharmacol Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.
| ||
Biochim Biophys Acta Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.
| ||
mBio SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense
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