MAGEH1 Polyclonal antibody
MAGEH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 12424-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Y79 cells, mouse brain tissue, PC-3 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
12424-1-AP targets MAGEH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MAGEH1 fusion protein Ag3090 相同性解析による交差性が予測される生物種 |
Full Name | melanoma antigen family H, 1 |
Calculated molecular weight | 219 aa, 24 kDa |
Observed molecular weight | 28 kDa |
GenBank accession number | BC011954 |
Gene symbol | MAGEH1 |
Gene ID (NCBI) | 28986 |
RRID | AB_2877856 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for MAGEH1 antibody 12424-1-AP | Download protocol |
IHC protocol for MAGEH1 antibody 12424-1-AP | Download protocol |
IF protocol for MAGEH1 antibody 12424-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway. |