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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGI2 Polyclonal antibody

MAGI2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25189-1-AP

Synonyms

ARIP1, AIP-1, AIP1, AIP 1, ACVRIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGI2 Polyclonal antibody (25189-1-AP)

    WB analysis using 25189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25189-1-AP (MAGI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAGI2 Polyclonal antibody (25189-1-AP)

    WB analysis using 25189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25189-1-AP (MAGI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using MAGI2 Polyclonal antibody (25189-1-AP)

    IF Staining of U-87 MG using 25189-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAGI2 antibody (25189-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25189-1-AP targets MAGI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGI2 fusion protein Ag18279 相同性解析による交差性が予測される生物種
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 2
Calculated molecular weight 1455 aa, 159 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC150277
Gene symbol MAGI2
Gene ID (NCBI) 9863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.

Protocols

Product Specific Protocols
WB protocol for MAGI2 antibody 25189-1-APDownload protocol
IF protocol for MAGI2 antibody 25189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols