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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGI2 Polyclonal antibody

MAGI2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25189-1-AP

Synonyms

ARIP1, AIP-1, AIP1, AIP 1, ACVRIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGI2 Polyclonal antibody (25189-1-AP)

    WB analysis using 25189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25189-1-AP (MAGI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAGI2 Polyclonal antibody (25189-1-AP)

    WB analysis using 25189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25189-1-AP (MAGI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using MAGI2 Polyclonal antibody (25189-1-AP)

    IF Staining of U-87 MG using 25189-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAGI2 antibody (25189-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25189-1-AP targets MAGI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGI2 fusion protein Ag18279 相同性解析による交差性が予測される生物種
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 2
Calculated molecular weight 1455 aa, 159 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC150277
Gene symbol MAGI2
Gene ID (NCBI) 9863
RRIDAB_3085775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.

Protocols

Product Specific Protocols
WB protocol for MAGI2 antibody 25189-1-APDownload protocol
IF protocol for MAGI2 antibody 25189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols