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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGI2 Recombinant antibody

MAGI2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240512C10

Cat no : 83561-6-RR

Synonyms

AIP-1, AIP1, AIP 1, ACVRIP1, ACVRINP1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using MAGI2 Recombinant antibody (83561-6-RR)

    WB analysis of rat brain using 83561-6-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 83561-6-RR (MAGI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83561-6-RR

    Affinity and Kinetic Characterization of 83561-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83561-6-RR against Human MAGI2 were performed. The affinity constant is 1.90 nM.

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Tested Applications

Positive WB detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83561-6-RR is MAGI2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGI2 fusion protein Ag18279 相同性解析による交差性が予測される生物種
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 2
Calculated molecular weight 1455 aa, 159 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC150277
Gene symbol MAGI2
Gene ID (NCBI) 9863
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.

Protocols

Product Specific Protocols
WB protocol for MAGI2 antibody 83561-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols