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  • Featured Product
  • KD/KO Validated

MAGT1 Polyclonal antibody

MAGT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17430-1-AP

Synonyms

magnesium transporter 1, Implantation-associated protein, IAP, IAG2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGT1 Polyclonal antibody (17430-1-AP)

    WB analysis using 17430-1-AP

    Mouse cerebellum lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAGT1 Polyclonal antibody (17430-1-AP)

    WB analysis using 17430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    WB analysis of mouse heart using 17430-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    WB analysis of mouse colon using 17430-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using MAGT1 Polyclonal antibody (17430-1-AP)

    WB analysis of COLO 320 using 17430-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAGT1 Polyclonal antibody (17430-1-AP)

    WB analysis of HEK-293 using 17430-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of human liver using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of human liver using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of mouse brain using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of mouse brain using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of human colon cancer using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17430-1-AP (MAGT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of human skeletal muscle using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MAGT1 Polyclonal antibody (17430-1-AP)

    IHC staining of human skeletal muscle using 17430-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAGT1 Polyclonal antibody (17430-1-AP)

    IF Staining of HepG2 using 17430-1-AP

    Immunofluorescent analysis of HepG2 cells, using 17430-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using MAGT1 Polyclonal antibody (17430-1-AP)

    IF Staining of C2C12 using 17430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MAGT1 antibody (17430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, COLO 320 cells, HEK-293 cells, mouse colon tissue, mouse heart tissue, rat cerebellum tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17430-1-AP targets MAGT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGT1 fusion protein Ag11496 相同性解析による交差性が予測される生物種
Full Name magnesium transporter 1
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC060842
Gene symbol MAGT1
Gene ID (NCBI) 84061
RRIDAB_2138982
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients'cells by western blot or immunofluorescent cell surface staining. MAGT1 had been detected as 38 kDa (PMID: 18705540) or 45-47 kDa (PMID: 27383987) by western blot.

Protocols

Product Specific Protocols
WB protocol for MAGT1 antibody 17430-1-APDownload protocol
IHC protocol for MAGT1 antibody 17430-1-APDownload protocol
IF protocol for MAGT1 antibody 17430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

Second messenger role for Mg2+ revealed by human T-cell immunodeficiency.

Authors - Li Feng-Yen FY
View Article
humanWB

Nature

Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.

Authors - Caleb D Marceau
  • KO Validated
View Article
ratWB,IF

Adv Sci (Weinh)

A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration.

Authors - Sihan Lin
View Article
ratWB,IF

Small

Magnetic Field Boosts the Transmembrane Transport Efficiency of Magnesium Ions from PLLA Bone Scaffold

Authors - Zuyun Yan
View Article
humanWB, IF

J Cell Biol

Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.

Authors - Natalia A Cherepanova
  • KD Validated
View Article
humanWB

MBio

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.

Authors - David L Lin
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Natalia (Verified Customer) (09-26-2019)

To my knowledge, this one is the only one commercially available antibody that works well for WB application to detect MagT1. It cross-reacts with TUSC3 a little, only because these are highly homological proteins.

  • Applications: Western Blot, Immunofluorescence,