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MAGT1 Polyclonal antibody
MAGT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17430-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse cerebellum tissue, COLO 320 cells, HEK-293 cells, mouse colon tissue, mouse heart tissue, rat cerebellum tissue |
Positive IHC detected in | human liver tissue, human colon cancer tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 17 publications below |
IF | See 5 publications below |
Product Information
17430-1-AP targets MAGT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MAGT1 fusion protein Ag11496 相同性解析による交差性が予測される生物種 |
Full Name | magnesium transporter 1 |
Calculated molecular weight | 335 aa, 38 kDa |
Observed molecular weight | 45-47 kDa |
GenBank accession number | BC060842 |
Gene symbol | MAGT1 |
Gene ID (NCBI) | 84061 |
RRID | AB_2138982 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients'cells by western blot or immunofluorescent cell surface staining. MAGT1 had been detected as 38 kDa (PMID: 18705540) or 45-47 kDa (PMID: 27383987) by western blot.
Protocols
Product Specific Protocols | |
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WB protocol for MAGT1 antibody 17430-1-AP | Download protocol |
IHC protocol for MAGT1 antibody 17430-1-AP | Download protocol |
IF protocol for MAGT1 antibody 17430-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
| ||
Adv Sci (Weinh) A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration. | ||
Small Magnetic Field Boosts the Transmembrane Transport Efficiency of Magnesium Ions from PLLA Bone Scaffold | ||
J Cell Biol Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
| ||
MBio Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Natalia (Verified Customer) (09-26-2019) | To my knowledge, this one is the only one commercially available antibody that works well for WB application to detect MagT1. It cross-reacts with TUSC3 a little, only because these are highly homological proteins.
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