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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MALT1 Monoclonal antibody (66225-1-Ig)

    WB analysis of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MALT1 Monoclonal antibody (66225-1-Ig)

    WB analysis of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MALT1 Monoclonal antibody (66225-1-Ig)

    WB analysis of Jurkat using 66225-1-Ig (same clone as 66225-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using MALT1 Monoclonal antibody (66225-1-Ig)

    WB analysis of K-562 using 66225-1-Ig (same clone as 66225-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MALT1 Monoclonal antibody (66225-1-Ig)

    IHC staining of human lymphoma using 66225-1-Ig (same clone as 66225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MALT1 Monoclonal antibody (66225-1-Ig)

    IHC staining of human lymphoma using 66225-1-Ig (same clone as 66225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MALT1 Monoclonal antibody (66225-1-Ig)

    IF Staining of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66225-1-Ig(MALT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

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MALT1 Monoclonal antibody, PBS Only

MALT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66225-1-PBS
Clone No.4D3F6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp434L132, MALT1, MLT, MLT1, Paracaspase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66225-1-PBS targets MALT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MALT1 fusion protein Ag20632 相同性解析による交差性が予測される生物種
Full Name mucosa associated lymphoid tissue lymphoma translocation gene 1
Calculated molecular weight 824 aa, 92 kDa
Observed molecular weight 92-100 kDa
GenBank accession numberBC030143
Gene Symbol MALT1
Gene ID (NCBI) 10892
RRIDAB_2881616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UDY8
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.