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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAML3 Polyclonal antibody (23263-1-AP)

    WB analysis of HEK-293 using 23263-1-AP (same clone as 23263-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MAML3 Polyclonal antibody (23263-1-AP)

    WB analysis of HEK-293 using 23263-1-AP (same clone as 23263-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MAML3 Polyclonal antibody (23263-1-AP)

    IF Staining of HEK-293 using 23263-1-AP (same clone as 23263-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 23263-1-AP (MAML3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23263-1-PBS in a different storage buffer formulation.

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MAML3 Polyclonal antibody, PBS Only

MAML3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23263-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23263-1-PBS targets MAML3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAML3 fusion protein Ag19788 相同性解析による交差性が予測される生物種
Full Name mastermind-like 3 (Drosophila)
Calculated molecular weight 1133 aa, 122 kDa
Observed molecular weight 122 kDa
GenBank accession numberBC137131
Gene Symbol MAML3
Gene ID (NCBI) 55534
RRIDAB_2879242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JK9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.