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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN1A1 Polyclonal antibody

MAN1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 23382-1-AP

Synonyms

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA, Man9-mannosidase, Man(9)-alpha-mannosidase, EC:3.2.1.113, Alpha-1,2-mannosidase IA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAN1A1 Polyclonal antibody (23382-1-AP)

    WB analysis using 23382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23382-1-AP (MAN1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using MAN1A1 Polyclonal antibody (23382-1-AP)

    IP experiment of MCF-7 using 23382-1-AP

    IP result of anti-MAN1A1 (IP:23382-1-AP, 4ug; Detection:23382-1-AP 1:300) with MCF-7 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MAN1A1 Polyclonal antibody (23382-1-AP)

    IHC staining of human ovary cancer using 23382-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23382-1-AP (MAN1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAN1A1 Polyclonal antibody (23382-1-AP)

    IF Staining of HepG2 using 23382-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (23382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using MAN1A1 Polyclonal antibody (23382-1-AP)

    FC experiment of HepG2 using 23382-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAN1A1 Polyclonal antibody (23382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23382-1-AP targets MAN1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAN1A1 fusion protein Ag20060 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 1A, member 1
Calculated molecular weight 653 aa, 73 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC065827
Gene symbol MAN1A1
Gene ID (NCBI) 4121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

Protocols

Product Specific Protocols
WB protocol for MAN1A1 antibody 23382-1-APDownload protocol
IHC protocol for MAN1A1 antibody 23382-1-APDownload protocol
IF protocol for MAN1A1 antibody 23382-1-APDownload protocol
IP protocol for MAN1A1 antibody 23382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (01-13-2023)

This antibody gave a fairly good staining at the Golgi area; however, it also stained microtubules, which is supposed to be nonspecific.

  • Applications: Immunofluorescence