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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAN1A1 Polyclonal antibody (23382-1-AP)

    WB analysis using 23382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23382-1-AP (MAN1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using MAN1A1 Polyclonal antibody (23382-1-AP)

    IP experiment of MCF-7 using 23382-1-AP

    IP result of anti-MAN1A1 (IP:23382-1-AP, 4ug; Detection:23382-1-AP 1:300) with MCF-7 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MAN1A1 Polyclonal antibody (23382-1-AP)

    IHC staining of human ovary cancer using 23382-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23382-1-AP (MAN1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAN1A1 Polyclonal antibody (23382-1-AP)

    IF Staining of HepG2 using 23382-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (23382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using MAN1A1 Polyclonal antibody (23382-1-AP)

    FC experiment of HepG2 using 23382-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAN1A1 Polyclonal antibody (23382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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MAN1A1 Polyclonal antibody

MAN1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 23382-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA, Man9-mannosidase, Man(9)-alpha-mannosidase, EC:3.2.1.113, Alpha-1,2-mannosidase IA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23382-1-AP targets MAN1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAN1A1 fusion protein Ag20060 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 1A, member 1
Calculated molecular weight 653 aa, 73 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC065827
Gene Symbol MAN1A1
Gene ID (NCBI) 4121
RRIDAB_2935458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

Protocols

Product Specific Protocols
WB protocol for MAN1A1 antibody 23382-1-APDownload protocol
IHC protocol for MAN1A1 antibody 23382-1-APDownload protocol
IF protocol for MAN1A1 antibody 23382-1-APDownload protocol
IP protocol for MAN1A1 antibody 23382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols