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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN2A1 Polyclonal antibody

MAN2A1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21132-1-AP

Synonyms

Alpha mannosidase 2, AMan II, Golgi alpha mannosidase II, GOLIM7, Man II, MAN2A1, MANA2, MANII

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach tissue using MAN2A1 Polyclonal antibody (21132-1-AP)

    IHC staining of human stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MAN2A1 Polyclonal antibody (21132-1-AP)

    IHC staining of human stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using MAN2A1 Polyclonal antibody (21132-1-AP)

    IHC staining of mouse stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using MAN2A1 Polyclonal antibody (21132-1-AP)

    IHC staining of mouse stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using MAN2A1 Polyclonal antibody (21132-1-AP)

    IHC staining of mouse stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAN2A1 Polyclonal antibody (21132-1-AP)

    IF Staining of HeLa using 21132-1-AP

    Immunofluorescent analysis of HeLa cells using 21132-1-AP (MAN2A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21132-1-AP targets MAN2A1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAN2A1 fusion protein Ag15536 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 2A, member 1
Calculated molecular weight 1144 aa, 131 kDa
GenBank accession numberBC142656
Gene symbol MAN2A1
Gene ID (NCBI) 4124
RRIDAB_2878816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MAN2A1 antibody 21132-1-APDownload protocol
IF protocol for MAN2A1 antibody 21132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (09-01-2020)

This antibody labels clean doublet bands around the expected molecular weight.

  • Applications: Western Blot