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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN2C1 Polyclonal antibody

MAN2C1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19189-1-AP

Synonyms

Alpha mannosidase 2C1, Alpha mannosidase 6A8B, MAN2C1, MAN6A8, MANA, MANA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAN2C1 Polyclonal antibody (19189-1-AP)

    WB analysis using 19189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19189-1-AP (MAN2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19189-1-AP targets MAN2C1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAN2C1 fusion protein Ag6284 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 2C, member 1
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC050550
Gene symbol MAN2C1
Gene ID (NCBI) 4123
RRIDAB_3669307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha mannosidase 2C1(MAN2C1) an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol. MAN2C1 cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues on cytoplasmatic free oligosaccharides generated by N-glycoprotein degradation pathways (PMID: 16848760). Impaired catabolism of free oligosaccharides due to MAN2C1 variants causes a neurodevelopmental disorder (PMID: 35045343).

Protocols

Product Specific Protocols
WB protocol for MAN2C1 antibody 19189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols