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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN2C1 Polyclonal antibody

MAN2C1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19189-1-AP

Synonyms

Alpha mannosidase 2C1, Alpha mannosidase 6A8B, MAN2C1, MAN6A8, MANA, MANA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAN2C1 Polyclonal antibody (19189-1-AP)

    WB analysis using 19189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19189-1-AP (MAN2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19189-1-AP targets MAN2C1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAN2C1 fusion protein Ag6284 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 2C, member 1
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC050550
Gene symbol MAN2C1
Gene ID (NCBI) 4123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha mannosidase 2C1(MAN2C1) an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol. MAN2C1 cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues on cytoplasmatic free oligosaccharides generated by N-glycoprotein degradation pathways (PMID: 16848760). Impaired catabolism of free oligosaccharides due to MAN2C1 variants causes a neurodevelopmental disorder (PMID: 35045343).

Protocols

Product Specific Protocols
WB protocol for MAN2C1 antibody 19189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols