MANEA Polyclonal antibody

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21296-1-AP

Synonyms

ENDO, Endo alpha mannosidase, Endomannosidase, hEndo, Mandaselin, MANEA, mannosidase, endo alpha


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Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21296-1-AP is MANEA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MANEA fusion protein Ag15847 相同性解析による交差性が予測される生物種
Full Name mannosidase, endo-alpha
Calculated molecular weight 462 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC146671
Gene symbol MANEA
Gene ID (NCBI) 79694
RRIDAB_2878836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MANEA antibody 21296-1-APDownload protocol
IHC protocol for MANEA antibody 21296-1-APDownload protocol
IF protocol for MANEA antibody 21296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols