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MAOA Polyclonal antibody

Name: MAOA Polyclonal antibody
Brand: Proteintech
SKU: 10539-1-AP

MAOA Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10539-1-AP

Synonyms

monoamine oxidase A, MAO-A, MAO A, EC:1.4.3.21, Amine oxidase [flavin-containing] A

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of LNCaP using 10539-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10539-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10539-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 10539-1-AP
IP result of anti-MAOA (IP:10539-1-AP, 3ug; Detection:10539-1-AP 1:600) with human placenta tissue lysate 2800ug.
IHC staining of human pancreas cancer using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 10539-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 10539-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	LNCaP cells, human placenta tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human pancreas cancer tissue, human kidney tissue, human liver tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 21 publications below
IHC	See 9 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

10539-1-AP targets MAOA in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAOA fusion protein Ag0821 相同性解析による交差性が予測される生物種
Full Name	monoamine oxidase A
Calculated molecular weight	60 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC008064
Gene symbol	MAOA
Gene ID (NCBI)	4128
RRID	AB_2137251
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Protocols

Product Specific Protocols
WB protocol for MAOA antibody 10539-1-AP	Download protocol
IHC protocol for MAOA antibody 10539-1-AP	Download protocol
IP protocol for MAOA antibody 10539-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell In situ identification of cellular drug targets in mammalian tissue. Authors - Zhengyuan Pang View Article
human	WB	Sci Adv p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Authors - Xiaochao Tan View Article
	WB	Sci Adv Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1. Authors - Odetta Antico View Article
		J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. Authors - Xiaochao Tan View Article
human	WB,IHC	J Hepatol Monoamine oxidase A suppresses hepatocellular carcinoma metastasis by inhibiting the adrenergic system and its transactivation of EGFR signaling. Authors - Jun Li View Article
mouse	WB	Aging (Albany NY) A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients. Authors - Xiao Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAOA Polyclonal antibody

MAOA Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of LNCaP using 10539-1-AP

WB analysis of human placenta using 10539-1-AP

WB analysis of human placenta using 10539-1-AP

IP experiment of human placenta using 10539-1-AP

IHC staining of human pancreas cancer using 10539-1-AP

IHC staining of human pancreas cancer using 10539-1-AP

IHC staining of human pancreas cancer using 10539-1-AP

IHC staining of human pancreas cancer using 10539-1-AP

IHC staining of human pancreas cancer using 10539-1-AP

IHC staining of human pancreas using 10539-1-AP

IHC staining of human pancreas using 10539-1-AP

IHC staining of human kidney using 10539-1-AP

IHC staining of human kidney using 10539-1-AP

IHC staining of human liver using 10539-1-AP

IHC staining of human liver using 10539-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

In situ identification of cellular drug targets in mammalian tissue.

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.

Monoamine oxidase A suppresses hepatocellular carcinoma metastasis by inhibiting the adrenergic system and its transactivation of EGFR signaling.

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.