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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAOA Monoclonal antibody

MAOA Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A9E4

Cat no : 68471-1-Ig

Synonyms

monoamine oxidase A, MAO-A, MAO A, EC:1.4.3.21, Amine oxidase [flavin-containing] A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAOA Monoclonal antibody (68471-1-Ig)

    WB analysis using 68471-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using MAOA Monoclonal antibody (68471-1-Ig)

    WB analysis of U2OS using 68471-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using MAOA Monoclonal antibody (68471-1-Ig)

    WB analysis of LNCaP using 68471-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Monoclonal antibody (68471-1-Ig)

    IHC staining of human pancreas cancer using 68471-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Monoclonal antibody (68471-1-Ig)

    IHC staining of human pancreas cancer using 68471-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using MAOA Monoclonal antibody (68471-1-Ig)

    FC experiment of HepG2 using 68471-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAOA Monoclonal antibody (68471-1-Ig, Clone:1A9E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inK-562 cells, U2OS cells, LNCaP cells, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68471-1-Ig targets MAOA in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAOA fusion protein Ag30028 相同性解析による交差性が予測される生物種
Full Name monoamine oxidase A
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC008064
Gene symbol MAOA
Gene ID (NCBI) 4128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Protocols

Product Specific Protocols
WB protocol for MAOA antibody 68471-1-IgDownload protocol
IHC protocol for MAOA antibody 68471-1-IgDownload protocol
FC protocol for MAOA antibody 68471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols