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LC3 Polyclonal antibody

Name: LC3 Polyclonal antibody
Brand: Proteintech
SKU: 14600-1-AP
Rating: 4.36 (11 reviews)

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

1272

Reviews (11)

Cat no : 14600-1-AP

Synonyms

MAP1LC3B, MAP1A/MAP1B LC3 B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 14600-1-AP
Chloroquine treated HepG2 cells (left) and untreated HepG2 cells (right) after serum deprived for 2 hours were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14600-1-AP
WB result of LC3 antibody (14600-1-AP; 1:6000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells.
WB analysis of HeLa using 14600-1-AP
WB result of LC3 antibody (14600-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LC3 transfected HeLa cells.
WB analysis of mouse heart using 14600-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14600-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14600-1-AP
CQ treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14600-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14600-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14600-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 14600-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 14600-1-AP
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14600-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 14600-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 14600-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14600-1-AP (LC3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14600-1-AP (LC3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14600-1-AP (LC3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14600-1-AP (LC3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 14600-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 14600-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 14600-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 14600-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human LC3 (14600-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 14600-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Chloroquine treated HepG2 cells, mouse brain tissue, mouse lung tissue, mouse testis tissue, human skeletal muscle tissue, rat brain tissue, Chloroquine treated HEK-293 cells, mouse heart tissue, mouse liver tissue, HeLa cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected in	human liver tissue, human testis tissue, mouse brain tissue, human breast cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1133 publications below
IHC	See 129 publications below
IF	See 354 publications below
IP	See 6 publications below
ELISA	See 1 publications below
CoIP	See 4 publications below

Product Information

14600-1-AP targets LC3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, monkey, chicken, zebrafish, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LC3 fusion protein Ag6144 相同性解析による交差性が予測される生物種
Full Name	microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight	15 kDa
Observed molecular weight	14-18 kDa
GenBank accession number	BC067797
Gene symbol	LC3B
Gene ID (NCBI)	81631
RRID	AB_2137737
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protocols

Product Specific Protocols
WB protocol for LC3 antibody 14600-1-AP	Download protocol
IHC protocol for LC3 antibody 14600-1-AP	Download protocol
IF protocol for LC3 antibody 14600-1-AP	Download protocol
FC protocol for LC3 antibody 14600-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy Authors - Yang Meng View Article
human	IF	Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). Authors - Marine H Laporte View Article
mouse	WB	Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells Authors - Decai Wang View Article
mouse	IF,WB	Bioact Mater Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway Authors - Tian Qin View Article
human	WB	Acta Neuropathol C9orf72 intermediate repeats are associated with corticobasal degeneration, increased C9orf72 expression and disruption of autophagy. Authors - Christopher P Cali View Article
mouse	WB,IF	Adv Sci (Weinh) DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion Authors - Xulong Ding View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Aditya (Verified Customer) (11-01-2024)	great with ecl Applications: Western Blot
FH David (Verified Customer) (01-02-2024)	Good antibody, single band at the predicted molecular weight, no background. Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: SH-SY5Y lysate
FH Hala (Verified Customer) (05-09-2023)	works very good in milk5% Applications: Western Blot Primary Antibody Dilution: 1/2000 Cell Tissue Type: HepG2 LC3 Antibody Western Blot validation (1/2000 dilution) in HepG2 (Cat no:14600-1-AP)
FH Hala (Verified Customer) (04-28-2023)	works very good with milk blocking Applications: Western Blot Primary Antibody Dilution: 1/2000 Cell Tissue Type: HepG2
FH Zhihao (Verified Customer) (07-25-2022)	We could detect one form of LC3 in our cell samples, which should be two. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: GFP-Parkin transfected HeLa cell LC3 Antibody Western Blot validation (1:1000 dilution) in GFP-Parkin transfected HeLa cell (Cat no:14600-1-AP)
FH S (Verified Customer) (01-01-2022)	I have tried three different LC3B antibodies from three different companies so far. This antibody is by far the best (cost efficient as well) Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: HCT116 LC3 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:14600-1-AP)
FH YING (Verified Customer) (09-25-2021)	The antibody works very well. Distinguished LC3-I and LC3-II bands. An increase of LC3-II level in response to treatment with bafilomycin A. Applications: Western Blot Primary Antibody Dilution: 1:1500 Cell Tissue Type: mouse cells LC3 Antibody Western Blot validation (1:1500 dilution) in mouse cells (Cat no:14600-1-AP)
FH Eric (Verified Customer) (03-14-2021)	Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested. Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Microglia LC3 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia (Cat no:14600-1-AP)
FH Elzbieta (Verified Customer) (09-23-2020)	The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mouse liver, human HepG2 cells
FH An (Verified Customer) (09-17-2020)	Used for IHC on zebrafish cryosections of eyes. Worked well. Applications: Immunohistochemistry
FH David (Verified Customer) (03-30-2020)	Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A. Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: SH-SY5Y

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LC3 Polyclonal antibody

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 14600-1-AP

WB analysis of HeLa using 14600-1-AP

WB analysis of HeLa using 14600-1-AP

WB analysis of mouse heart using 14600-1-AP

WB analysis of mouse liver using 14600-1-AP

WB analysis of HEK-293 using 14600-1-AP

WB analysis of HeLa using 14600-1-AP

WB analysis of rat brain using 14600-1-AP

WB analysis of mouse testis using 14600-1-AP

WB analysis of human skeletal muscle using 14600-1-AP

WB analysis of HSC-T6 using 14600-1-AP

WB analysis of mouse brain using 14600-1-AP

WB analysis of mouse lung using 14600-1-AP

WB analysis of NIH/3T3 using 14600-1-AP

IHC staining of human liver using 14600-1-AP

IHC staining of human liver using 14600-1-AP

IHC staining of human gliomas using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of human gliomas using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of human testis using 14600-1-AP

IHC staining of human testis using 14600-1-AP

IHC staining of human breast cancer using 14600-1-AP

IHC staining of human breast cancer using 14600-1-AP

IF Staining of HeLa using 14600-1-AP

IF Staining of HepG2 using 14600-1-AP

IF Staining of HepG2 using 14600-1-AP

FC experiment of HeLa using 14600-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway

C9orf72 intermediate repeats are associated with corticobasal degeneration, increased C9orf72 expression and disruption of autophagy.

DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion

Reviews

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