Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Chloroquine treated HeLa cells |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 369 publications below |
| IHC | See 63 publications below |
| IF | See 137 publications below |
| IP | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
18725-1-AP targets LC3B-Specific in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule-associated protein 1 light chain 3 beta |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15 kDa, 18 kDa |
| GenBank accession number | NM_022818 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_2137745 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
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Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| IF protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| IHC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| WB protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models. | ||
Am J Respir Crit Care Med Acid Sphingomyelinase Inhibition Attenuates Cell Death in Mechanically-Ventilated Newborn Rat Lung. | ||
J Extracell Vesicles Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles. | ||
Nat Neurosci The mitochondrial unfolded protein response in human microglia disrupts neuronal-glial communication and promotes senescence. | ||
Autophagy Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival. |