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- KD/KO Validated
MAP1S Polyclonal antibody
MAP1S Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15695-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human prostate cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
15695-1-AP targets MAP1S in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAP1S fusion protein Ag8315 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule-associated protein 1S |
| Calculated molecular weight | 806 aa, 85 kDa |
| Observed molecular weight | 130-150 kDa |
| GenBank accession number | BC008806 |
| Gene symbol | MAP1S |
| Gene ID (NCBI) | 55201 |
| RRID | AB_2137868 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP1S (also known as C19ORF5 or VCY2IP1) is a novel member of the microtubule-associated protein 1 family and a homologue of the exclusively neuronal distributed microtubule-associated protein 1A and 1B (MAP1A/B). In contrast to MAP1A and MAP1B, MAP1S is expressed in a wide range of tissues in addition to neurons. MAP1S is synthesized as a precursor protein that is partially cleaved into heavy and light chains in a tissue-specific manner. In addition, a short chain isoform may be induced under prolonged mitotic arrest or inhibition of the 26S proteasome. Recently it has been reported that the short chain isoform associates with mitochondria in addition to microtubules and causes irreversible aggregation of dysfunctional mitochondria resulting in cell death. Western blot analysis in human brain using this antibody detected two main bands between 100-130 kDa corresponding to heavy and light chains of MAP1S.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP1S antibody 15695-1-AP | Download protocol |
| IHC protocol for MAP1S antibody 15695-1-AP | Download protocol |
| IF protocol for MAP1S antibody 15695-1-AP | Download protocol |
| IP protocol for MAP1S antibody 15695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. | ||
EMBO Rep MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules. | ||
Cell Cycle Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability. | ||
Front Synaptic Neurosci Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction. | ||
Biochim Biophys Acta Mol Cell Res Cryptic splicing events result in unexpected protein products from calpain-10 (CAPN10) cDNA |
