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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP2 Recombinant antibody

MAP2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241653H8

Cat no : 84306-4-RR

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP2 Recombinant antibody (84306-4-RR)

    WB analysis using 84306-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84306-4-RR (MAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using MAP2 Recombinant antibody (84306-4-RR)

    IF Staining of SH-SY5Y using 84306-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inSH-SY5Y cells, U-251 cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84306-4-RR targets MAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAP2 fusion protein Ag11580 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 2
Calculated molecular weight 200 kDa
Observed molecular weight280-300 kDa, 70-85 kDa
GenBank accession numberBC038857
Gene symbol MAP2
Gene ID (NCBI) 4133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

Protocols

Product Specific Protocols
WB protocol for MAP2 antibody 84306-4-RRDownload protocol
IF protocol for MAP2 antibody 84306-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols