MEK1/2 Polyclonal antibody

MEK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11049-1-AP

Synonyms

MAP2K2, MEK2, MAPK/ERK kinase 2, MAP kinase kinase 2, ERK activator kinase 2


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Tested Applications

Positive WB detected inA549 cells, human brain tissue, HL-60 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inHL-60 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11049-1-AP targets MEK1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK1/2 fusion protein Ag1481 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC018645
Gene symbol MEK2
Gene ID (NCBI) 5605
RRIDAB_2140649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.

Protocols

Product Specific Protocols
WB protocol for MEK1/2 antibody 11049-1-APDownload protocol
IHC protocol for MEK1/2 antibody 11049-1-APDownload protocol
IF protocol for MEK1/2 antibody 11049-1-APDownload protocol
IP protocol for MEK1/2 antibody 11049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Mol Med

Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer

Authors - Yanmei Peng
mouseWB

Nat Commun

ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.

Authors - Yan Wang
mouseWB

ACS Appl Mater Interfaces

Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.

Authors - Chaoqun Li
mouse,humanWB

Cell Rep

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Authors - Hao Qin
humanWB

J Med Chem

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Authors - Zongyuan Zhou
humanWB

Cell Death Dis

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Authors - Wenjuan Li