MEK1/2 Polyclonal antibody
MEK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11049-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, human brain tissue, HL-60 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HL-60 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Jurkat cells, SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 56 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
11049-1-AP targets MEK1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MEK1/2 fusion protein Ag1481 相同性解析による交差性が予測される生物種 |
Full Name | mitogen-activated protein kinase kinase 2 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 44-47 kDa |
GenBank accession number | BC018645 |
Gene symbol | MEK2 |
Gene ID (NCBI) | 5605 |
RRID | AB_2140649 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.
Protocols
Product Specific Protocols | |
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WB protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
IHC protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
IF protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
IP protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer | ||
ACS Appl Mater Interfaces Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels. | ||
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism | ||
J Med Chem Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma. | ||