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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEK1/2 Recombinant antibody, PBS Only (Capture/Detector)

MEK1/2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242614G3

Cat no : 84967-1-PBS

Synonyms

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEK1/2 Recombinant antibody (84967-1-RR)

    WB analysis using 84967-1-RR (same clone as 84967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84967-1-RR (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01692-1

    Sandwich ELISA standard curve of MP01692-1

    Sandwich ELISA standard curve of MP01692-1, Human MEK2 Recombinant Matched Antibody Pair - PBS only. 84967-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1481. 84967-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP01692-1

    Sample test of MP01692-1

    The mean MEK2 concentration was determined to be 45.61 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load and 44.07 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MEK1/2 Recombinant antibody (84967-1-RR)

    IF Staining of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (84967-1-RR, Clone: 242614G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01692-1

    Cytometric bead array standard curve of MP01692-1

    Cytometric bead array standard curve of MP01692-1, MEK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84967-2-PBS. Detection antibody: 84967-1-PBS. Standard: Ag1481. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01692-2

    Cytometric bead array standard curve of MP01692-2

    Cytometric bead array standard curve of MP01692-2, MEK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84967-1-PBS. Detection antibody: 84967-3-PBS. Standard: Ag1481. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84967-1-PBS targets MEK1/2 as part of a matched antibody pair:

MP01692-1: 84967-2-PBS capture and 84967-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01692-2: 84967-1-PBS capture and 84967-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MEK1/2 fusion protein Ag1481 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC018645
Gene symbol MEK2
Gene ID (NCBI) 5605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 84967-1-RR can recognize both the MEK2 and MEK1.