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KD/KO Validated

MEK3 Polyclonal antibody

Name: MEK3 Polyclonal antibody
Brand: Proteintech
SKU: 13898-1-AP

MEK3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13898-1-AP

Synonyms

MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3

Validation Data Gallery

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AllWBIPIHC

WB analysis of HeLa using 13898-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13898-1-AP (MEK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13898-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13898-1-AP (MEK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13898-1-AP
IP result of anti-MEK3 (IP:13898-1-AP, 4ug; Detection:13898-1-AP 1:1000) with HeLa cells lysate 3200ug.
IP experiment of HeLa using 13898-1-AP
IP result of anti-MEK3 (IP:13898-1-AP, 4ug; Detection:13898-1-AP 1:1000) with HeLa cells lysate 3200ug.
IHC staining of mouse skeletal muscle using 13898-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skeletal muscle using 13898-1-AP
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skeletal muscle using 13898-1-AP
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 13898-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 13898-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below

Product Information

13898-1-AP targets MEK3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MEK3 fusion protein Ag4861 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase 3
Calculated molecular weight	347 aa, 39 kDa
Observed molecular weight	37-40 kDa
GenBank accession number	BC032478
Gene symbol	MAP2K3
Gene ID (NCBI)	5606
RRID	AB_10642959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MEK3 antibody 13898-1-AP	Download protocol
IHC protocol for MEK3 antibody 13898-1-AP	Download protocol
IP protocol for MEK3 antibody 13898-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biomed Pharmacother Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells Authors - Ling Gu KD Validated View Article
human	WB	Cell Mol Life Sci HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress Authors - Joan Sala-Gaston View Article
mouse	WB	Food Funct L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice. Authors - Kehong Liu View Article
human	WB	Sci Rep The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling. Authors - Leonardo Pedrazza KD Validated View Article
mouse	WB	Mol Immunol Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism. Authors - Libo Hou View Article
human	WB	PLoS One Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis. Authors - Xu Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEK3 Polyclonal antibody

MEK3 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 13898-1-AP

WB analysis of HeLa using 13898-1-AP

IP experiment of HeLa using 13898-1-AP

IP experiment of HeLa using 13898-1-AP

IHC staining of mouse skeletal muscle using 13898-1-AP

IHC staining of rat skeletal muscle using 13898-1-AP

IHC staining of rat skeletal muscle using 13898-1-AP

IHC staining of mouse skeletal muscle using 13898-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells

HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress

L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.

The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.

Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.

Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.