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KD/KO Validated

MAP2K4 Polyclonal antibody

Name: MAP2K4 Polyclonal antibody
Brand: Proteintech
SKU: 17340-1-AP

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17340-1-AP

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

Validation Data Gallery

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AllWBIHC

WB analysis using 17340-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 17340-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17340-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 17340-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17340-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17340-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 17340-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 17340-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 17340-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

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Cat No. 17340-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 11 publications below
IHC	See 2 publications below

Product Information

17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAP2K4 fusion protein Ag11376 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase 4
Calculated molecular weight	399 aa, 44 kDa
Observed molecular weight	44-50 kDa
GenBank accession number	BC036032
Gene symbol	MAP2K4
Gene ID (NCBI)	6416
RRID	AB_2878389
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP2K4 antibody 17340-1-AP	Download protocol
IHC protocol for MAP2K4 antibody 17340-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	EBioMedicine PMID: 31594754 Authors - Yiyi Liu View Article
mouse,rat	WB	J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. Authors - Junrong Wu View Article
mouse	WB	J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. Authors - Ze-Qing Pu View Article
rat	WB	Cells Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning. Authors - Elaheh Soleimanpour View Article
human	WB	Int J Biochem Cell Biol Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression. Authors - Xuechao Wan KD Validated View Article
human	WB	Dig Dis Sci LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4. Authors - Jianhui Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP2K4 Polyclonal antibody

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 17340-1-AP

WB analysis of RAW264.7 using 17340-1-AP

WB analysis of HeLa using 17340-1-AP

IHC staining of human liver cancer using 17340-1-AP

IHC staining of human liver cancer using 17340-1-AP

IHC staining of human breast cancer using 17340-1-AP

IHC staining of human breast cancer using 17340-1-AP

IHC staining of human skeletal muscle using 17340-1-AP

IHC staining of human skeletal muscle using 17340-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PMID: 31594754

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.

Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.

Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.

LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.