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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP2K4 Monoclonal antibody, PBS Only

MAP2K4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E4C1

Cat no : 67333-1-PBS

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP2K4 Monoclonal antibody (67333-1-Ig)

    WB analysis using 67333-1-Ig (same clone as 67333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAP2K4 Monoclonal antibody (67333-1-Ig)

    IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAP2K4 Monoclonal antibody (67333-1-Ig)

    IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67333-1-PBS targets MAP2K4 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2K4 fusion protein Ag11348 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 4
Calculated molecular weight 399 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC036032
Gene symbol MAP2K4
Gene ID (NCBI) 6416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.