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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEK5 Polyclonal antibody

MEK5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15758-1-AP

Synonyms

MAP2K5, MKK5, MAPKK5, HsT17454, EC:2.7.12.2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using MEK5 Polyclonal antibody (15758-1-AP)

    WB analysis of HEK-293T using 15758-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using MEK5 Polyclonal antibody (15758-1-AP)

    WB analysis of A431 using 15758-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEK5 Polyclonal antibody (15758-1-AP)

    WB analysis of HeLa using 15758-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human heart using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human heart using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEK5 Polyclonal antibody (15758-1-AP)

    IF Staining of HeLa using 15758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK5 antibody (15758-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293T cells, A431 cells, HeLa cells
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15758-1-AP targets MEK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK5 fusion protein Ag8351 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 5
Calculated molecular weight 448 aa, 50 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC008838
Gene symbol MEK5
Gene ID (NCBI) 5607
RRIDAB_2141275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MEK5 antibody 15758-1-APDownload protocol
IHC protocol for MEK5 antibody 15758-1-APDownload protocol
IF protocol for MEK5 antibody 15758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Hyperthermia

Decrease in MAP3Ks expression enhances the cell death caused by hyperthermia.

Authors - Atsushi Enomoto
View Article
ratWB

Pharm Biol

Injection of YiQiFuMai powder protects against heart failure via inhibiting p38 and ERK1/2 MAPKs activation.

Authors - Yongwei Nie
View Article