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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MLK2 Polyclonal antibody

MLK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19974-1-AP

Synonyms

MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using MLK2 Polyclonal antibody (19974-1-AP)

    WB analysis of human skeletal muscle using 19974-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19974-1-AP (MLK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MLK2 Polyclonal antibody (19974-1-AP)

    WB analysis of human brain using 19974-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19974-1-AP (MLK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MLK2 Polyclonal antibody (19974-1-AP)

    IHC staining of human pancreas cancer using 19974-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MLK2 Polyclonal antibody (19974-1-AP)

    IHC staining of human pancreas cancer using 19974-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19974-1-AP targets MLK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 10
Calculated molecular weight 104 kDa
Observed molecular weight 104-115 kDa
GenBank accession numberNM_002446
Gene symbol MAP3K10
Gene ID (NCBI) 4294
RRIDAB_10732824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K10. The antibody has no cross reaction to MAP3K9.

Protocols

Product Specific Protocols
WB protocol for MLK2 antibody 19974-1-APDownload protocol
IHC protocol for MLK2 antibody 19974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Lab Chip

Paper-based microreactor array for rapid screening of cell signaling cascades.

Authors - Chia-Hao Huang
View Article
humanWB

J Biol Chem

Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division.

Authors - Luis Cedeno-Rosario
View Article