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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEKK2 Polyclonal antibody (29265-1-AP)

    WB analysis using 29265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29265-1-AP (MEKK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MEKK2 Polyclonal antibody (29265-1-AP)

    IHC staining of human breast cancer using 29265-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29265-1-AP (MEKK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MEKK2 Polyclonal antibody (29265-1-AP)

    IHC staining of human breast cancer using 29265-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29265-1-AP (MEKK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MEKK2 Polyclonal antibody

MEKK2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29265-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MAP3K2, EC:2.7.11.25, MEKK2B, MEKK 2, MEK kinase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 500 ug/ml
20 μL, 500 ug/ml
150 μL, 500 ug/ml

¥67,000/ 150 μL

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29265-1-AP targets MEKK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEKK2 fusion protein Ag30886 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 2
Calculated molecular weight 70 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_006609
Gene Symbol MAP3K2
Gene ID (NCBI) 10746
RRIDAB_2918263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2.

Protocols

Product Specific Protocols
WB protocol for MEKK2 antibody 29265-1-APDownload protocol
IHC protocol for MEKK2 antibody 29265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

Concurrent targeting of MAP3K3 and BRD4 by miR-3140-3p overcomes acquired resistance to BET inhibitors in neuroblastoma cells.

Authors - Chang Liu
View Article