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This product has been discontinued. We recommend 28201-1-AP as an alternative.
For more information, please contact us.


Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

14385-1-AP targets ASK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASK1 fusion protein Ag5818 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight 155 kDa
GenBank accession numberBC054503
Gene Symbol ASK1
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99683
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38.

Publications

SpeciesApplicationTitle
ratWB

J Mol Cell Cardiol

Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction.

Authors - Na Zhao
monkeyWB

Cell Microbiol

\Non-haemolytic enterotoxin (Nhe) of Bacillus cereus induces apoptosis in Vero cells.

Authors - Xiaoye Liu
humanWB

Life Sci

Thioredoxin-1 phosphorylated at T100 is needed for its anti-apoptotic activity in HepG2 cancer cells.

Authors - Chen Xiaoping X
ratWB

J Agric Food Chem

Ginsenoside F4 Alleviates Skeletal Muscle Insulin Resistance by Regulating PTP1B in Type II Diabetes Mellitus

Authors - Yujie Zhao
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