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  • KD/KO Validated

TAK1 Polyclonal antibody

TAK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, hamster and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12330-2-AP

Synonyms

MAP3K7, TGF beta activated kinase 1, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7


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Tested Applications

Positive WB detected inNIH/3T3 cells, K-562 cells, SKOV-3 cells, COLO 320 cells, A375 cells, A431 cells, CHO cells, MCF-7 cells, HeLa cells, mouse heart tissue, A2780 cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12330-2-AP targets TAK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, hamster samples.

Tested Reactivity human, mouse, hamster
Cited Reactivityhuman, mouse, rat, pig, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAK1 fusion protein Ag3041 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated molecular weight 579 aa, 64 kDa
Observed molecular weight 64-67 kDa, 75-80 kDa
GenBank accession numberBC017715
Gene symbol TAK1
Gene ID (NCBI) 6885
RRIDAB_2140101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7-mitogen-activated protein kinase kinase kinase 7-  is a serine/threonine  protein kinase triggering phosphorylation of AMP-activated protein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic  proteins (BMPs), and various proinflammatory mediators.


What is the molecular weight of TAK1? Are there any isoforms of TAK1?

The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-B represents the full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12 and/or 16. TAK-1 variants differ in their expression levels across tissues (PMID: 11118615).


Is TAK1 post-translationally modified?

TAK1 kinase activity is modulated by interacting with TAK1-binding proteins (TABs). Binding  to  TABs  triggers autophosphorylation of TAK1 and depends on  polyubiquitination of receptor-interacting protein 1 (RIP1) (PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated, which impacts its activity (PMID: 28106845).


What is the subcellular localization of TAK1?

TAK1 is present in the cytoplasm.


What is the tissue expression pattern of TAK1?

TAK1 is ubiquitously expressed.


How can TAK1 activity be monitored?

TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs on Thr178, Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187 phosphorylation.


Protocols

Product Specific Protocols
WB protocol for TAK1 antibody 12330-2-APDownload protocol
IHC protocol for TAK1 antibody 12330-2-APDownload protocol
IF protocol for TAK1 antibody 12330-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
  • KD Validated
mouseWB

Cell Death Differ

Protein phosphatase 6 is a key factor regulating spermatogenesis.

Authors - Wen-Long Lei
mouseWB

Diabetes

Hepatic P38 Activation Modulates Systemic Metabolism Through Fgf21-Mediated Interorgan Communication.

Authors - Wei Liu
humanWB

EMBO Rep

Mycobacterium tuberculosis protein kinase G acts as an unusual ubiquitinating enzyme to impair host immunity.

Authors - Jing Wang
mouseWB

PLoS Pathog

Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.

Authors - Gang Xu
mouseWB

Food Funct

DHA-phospholipids (DHA-PL) and EPA-phospholipids (EPA-PL) prevent intestinal dysfunction induced by chronic stress

Authors - Wanxiu Cao