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KD/KO Validated

TAB2 Polyclonal antibody

Name: TAB2 Polyclonal antibody
Brand: Proteintech
SKU: 14410-1-AP

TAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14410-1-AP

Synonyms

MAP3K7IP2, KIAA0733, TAK1 binding protein 2, TAB-2, TAB 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14410-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 14410-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 14410-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14410-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14410-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14410-1-AP
IP result of anti-TAB2 (IP:14410-1-AP, 4ug; Detection:14410-1-AP 1:600) with HepG2 cells lysate 3600ug.
IHC staining of human malignant melanoma using 14410-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human malignant melanoma using 14410-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 14410-1-AP
Immunofluorescent analysis of HepG2 cells, using MAP3K7IP2 antibody 14410-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14410-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	L02 cells, C6 cells, human liver tissue, HepG2 cells, mouse liver tissue, rat liver tissue, Raji cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human malignant melanoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 18 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

14410-1-AP targets TAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TAB2 fusion protein Ag5742 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase kinase 7 interacting protein 2
Calculated molecular weight	76 kDa
Observed molecular weight	77 kDa
GenBank accession number	BC035910
Gene symbol	MAP3K7IP2
Gene ID (NCBI)	23118
RRID	AB_2281638
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tab2 has a role in the inflammatory signal transduction pathway and is involved in the reversal of NCoR-dependent and NFkB- or APP-mediated gene repression and androgen antagonist response. Besides, it is identified as a cofactor for ERBB4 to assemble NCoR corepressor complex in response to neuregulin [PMID:16469706]. It also functions as an adaptor that links TAK1 and TRAF6 in response to interleukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α), and thereby mediates TAK1 activation [PMID:11460167].

Protocols

Product Specific Protocols
WB protocol for TAB2 antibody 14410-1-AP	Download protocol
IHC protocol for TAB2 antibody 14410-1-AP	Download protocol
IF protocol for TAB2 antibody 14410-1-AP	Download protocol
IP protocol for TAB2 antibody 14410-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell An Interaction Landscape of Ubiquitin Signaling. Authors - Xiaofei Zhang View Article
human	WB	Nat Commun Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. Authors - Chunjie Sheng View Article
human	WB	Cell Biol Toxicol microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro. Authors - Qiuyue Li KD Validated View Article
mouse	WB	Front Immunol MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byMycobacterium aviumsubspeciesparatuberculosisby Targeting Interleukin-10 and TGF-β-Activated Protein Kinase 1 Binding Protein 2. Authors - Tariq Hussain View Article
human	WB	J Neuroinflammation miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses. Authors - Bo Yang View Article
human	WB	Front Immunol Hepatitis B Surface Antigen Suppresses the Activation of Nuclear Factor Kappa B Pathway via Interaction With the TAK1-TAB2 Complex. Authors - Feiyan Deng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAB2 Polyclonal antibody

TAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14410-1-AP

WB analysis using 14410-1-AP

WB analysis of L02 using 14410-1-AP

WB analysis of HepG2 using 14410-1-AP

WB analysis of human liver using 14410-1-AP

IP experiment of HepG2 using 14410-1-AP

IHC staining of human malignant melanoma using 14410-1-AP

IHC staining of human malignant melanoma using 14410-1-AP

IF Staining of HepG2 using 14410-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An Interaction Landscape of Ubiquitin Signaling.

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.

MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byMycobacterium aviumsubspeciesparatuberculosisby Targeting Interleukin-10 and TGF-β-Activated Protein Kinase 1 Binding Protein 2.

miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses.

Hepatitis B Surface Antigen Suppresses the Activation of Nuclear Factor Kappa B Pathway via Interaction With the TAK1-TAB2 Complex.