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  • Featured Product
  • KD/KO Validated

MAP4 Polyclonal antibody, PBS Only

MAP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11229-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAP4 Polyclonal antibody (11229-1-AP)

    WB analysis of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAP4 Polyclonal antibody (11229-1-AP)

    WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C2C12 cells using MAP4 Polyclonal antibody (11229-1-AP)

    WB analysis of C2C12 using 11229-1-AP (same clone as 11229-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using MAP4 Polyclonal antibody (11229-1-AP)

    WB analysis of C6 using 11229-1-AP (same clone as 11229-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MAP4 Polyclonal antibody (11229-1-AP)

    IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

    IP result of anti-MAP4 (IP:11229-1-AP, 4ug; Detection:11229-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using MAP4 Polyclonal antibody (11229-1-AP)

    IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using MAP4 Polyclonal antibody (11229-1-AP)

    IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11229-1-PBS targets MAP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4 fusion protein Ag1741 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 4
Calculated molecular weight 121 kDa
Observed molecular weight 210-240 kDa
GenBank accession numberBC012794
Gene symbol MAP4
Gene ID (NCBI) 4134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)