MAP4K1 Polyclonal antibody

Name: MAP4K1 Polyclonal antibody
Brand: Proteintech
SKU: 23950-1-AP

MAP4K1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23950-1-AP

Synonyms

HPK1, MAP4K1, MEK kinase kinase 1, MEKKK 1

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of Raji using 23950-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 23950-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 23950-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 23950-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 23950-1-AP
IP result of anti-MAP4K1 (IP:23950-1-AP, 4ug; Detection:23950-1-AP 1:1000) with Jurkat cells lysate 3200ug.
IHC staining of human tonsillitis using 23950-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 23950-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 23950-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 23950-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 23950-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, Raji cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human tonsillitis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

23950-1-AP targets MAP4K1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAP4K1 fusion protein Ag20221 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase kinase kinase 1
Calculated molecular weight	833 aa, 91 kDa
Observed molecular weight	90 kDa
GenBank accession number	BC152994
Gene symbol	MAP4K1
Gene ID (NCBI)	11184
RRID	AB_2879372
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAP4K1(Mitogen-activated protein kinase kinase kinase kinase 1) is also named as MEKKK 1, HPK1 and belongs to the protein kinase superfamily. It may function as a novel tumor suppressor and its loss plays a critical role in pancreatic cancer. MAP4K1 regulates stress responses, proliferation, and apoptosis in hematopoietic cells. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP4K1 antibody 23950-1-AP	Download protocol
IHC protocol for MAP4K1 antibody 23950-1-AP	Download protocol
IP protocol for MAP4K1 antibody 23950-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Signal SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells. Authors - Xunping Zhao View Article
human	WB	Clin Exp Med An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma Authors - Lin Yang View Article
human	WB	Nat Commun DNA framework-engineered chimeras platform enables selectively targeted protein degradation Authors - Li Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP4K1 Polyclonal antibody

MAP4K1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of Raji using 23950-1-AP

WB analysis using 23950-1-AP

WB analysis of Jurkat using 23950-1-AP

WB analysis of Jurkat using 23950-1-AP

IP experiment of Jurkat using 23950-1-AP

IHC staining of human tonsillitis using 23950-1-AP

IHC staining of human tonsillitis using 23950-1-AP

IHC staining of human liver using 23950-1-AP