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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP4K2 Polyclonal antibody

MAP4K2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55244-1-AP

Synonyms

BL44, GC kinase, GCK, Germinal center kinase, MAP4K2, MEK kinase kinase 2, MEKKK 2, Rab8 interacting protein, RAB8IP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    WB analysis of mouse brain using 55244-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    WB analysis of human brain using 55244-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using MAP4K2 Polyclonal antibody (55244-1-AP)

    WB analysis of Raji using 55244-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human colon cancer using 55244-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human colon cancer using 55244-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human lung cancer using 55244-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human lung cancer using 55244-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, Raji cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55244-1-AP targets MAP4K2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase kinase 2
Calculated molecular weight 92 kDa
Observed molecular weight 85-91 kDa, 58 kDa
GenBank accession numberNM_004579
Gene symbol MAP4K2
Gene ID (NCBI) 5871
RRIDAB_10860908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP4K2,also named as GCK and RAB8IP, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. MAP4K2 may play a role in the regulation of vesicle targeting or fusion. This antibody is specific to MAP4K2.

Protocols

Product Specific Protocols
WB protocol for MAP4K2 antibody 55244-1-APDownload protocol
IHC protocol for MAP4K2 antibody 55244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols