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  • Featured Product
  • KD/KO Validated

MAP4K3 Polyclonal antibody

MAP4K3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14702-1-AP

Synonyms

GLK, MAP4K3, MAPKKKK3, MEK kinase kinase 3, MEKKK 3, RAB8IPL1

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using MAP4K3 Polyclonal antibody (14702-1-AP)

    WB analysis of human skeletal muscle using 14702-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MAP4K3 Polyclonal antibody (14702-1-AP)

    WB analysis of human brain using 14702-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAP4K3 Polyclonal antibody (14702-1-AP)

    WB analysis of HeLa using 14702-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MAP4K3 Polyclonal antibody (14702-1-AP)

    IHC staining of human skeletal muscle using 14702-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MAP4K3 Polyclonal antibody (14702-1-AP)

    IHC staining of human skeletal muscle using 14702-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAP4K3 Polyclonal antibody (14702-1-AP)

    IHC staining of human heart using 14702-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAP4K3 Polyclonal antibody (14702-1-AP)

    IHC staining of human heart using 14702-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HeLa cells, human brain tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14702-1-AP targets MAP4K3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4K3 fusion protein Ag6372 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase kinase 3
Calculated molecular weight 101 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC071579
Gene symbol MAP4K3
Gene ID (NCBI) 8491
RRIDAB_10858917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.

Protocols

Product Specific Protocols
WB protocol for MAP4K3 antibody 14702-1-APDownload protocol
IHC protocol for MAP4K3 antibody 14702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB

Bioengineered

Mitogen-activating protein kinase kinase kinase kinase-3, inhibited by Astragaloside IV through H3 lysine 4 monomethylation, promotes the progression of diabetic nephropathy by inducing apoptosis.

Authors - Yuyan Fan
  • KD Validated
View Article