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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP4K3 Polyclonal antibody

MAP4K3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30716-1-AP

Synonyms

MEKKK 3, MEK kinase kinase 3, MAPKKKK3, GLK, Germinal center kinase-related protein kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP4K3 Polyclonal antibody (30716-1-AP)

    WB analysis using 30716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30716-1-AP (MAP4K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MAP4K3 Polyclonal antibody (30716-1-AP)

    IHC staining of mouse skeletal muscle using 30716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MAP4K3 Polyclonal antibody (30716-1-AP)

    IHC staining of mouse skeletal muscle using 30716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using MAP4K3 Polyclonal antibody (30716-1-AP)

    IF Staining of U-87 MG using 30716-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAP4K3 antibody (30716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inU-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30716-1-AP targets MAP4K3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4K3 fusion protein Ag33512 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase kinase 3
Calculated molecular weight 101 kDa
Observed molecular weight 99-101 kDa
GenBank accession numberBC071579
Gene symbol MAP4K3
Gene ID (NCBI) 8491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.

Protocols

Product Specific Protocols
WB protocol for MAP4K3 antibody 30716-1-APDownload protocol
IHC protocol for MAP4K3 antibody 30716-1-APDownload protocol
IF protocol for MAP4K3 antibody 30716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols