☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

MAP4K4 Polyclonal antibody

MAP4K4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 55247-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

FLH21957, HGK, HPK/GCK like kinase HGK, KIAA0687, MAP4K4, MEK kinase kinase 4, MEKKK 4, Nck interacting kinase, NIK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue, human placenta tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase kinase 4
Calculated molecular weight 151 kDa
Observed molecular weight 130-160 kDa
GenBank accession numberNM_004834
Gene Symbol MAP4K4
Gene ID (NCBI) 9448
RRIDAB_10836939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

Protocols

Product Specific Protocols
WB protocol for MAP4K4 antibody 55247-1-APDownload protocol
IHC protocol for MAP4K4 antibody 55247-1-APDownload protocol
IP protocol for MAP4K4 antibody 55247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway

Authors - Lulu Chen
humanWB

Anal Chem

Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro.

Authors - Weili Miao
humanWB

EBioMedicine

Targeting miR-30d reverses pathological cardiac hypertrophy.

Authors - Jin Li
mouseWB

J Proteome Res

Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.

Authors - Jingzi Zhang
mouseWB,IF

Neural Regen Res

MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.

Authors - Zheng Zou
  • KD Validated
humanIHC

PeerJ

Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.

Authors - Wanzhen Li
Loading...