MAP7 Polyclonal antibody

MAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13446-1-AP

Synonyms

Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115


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Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13446-1-AP targets MAP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP7 fusion protein Ag4276 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 7
Calculated molecular weight 749 aa, 84 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025777
Gene symbol MAP7
Gene ID (NCBI) 9053
RRIDAB_10644485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAP7 antibody 13446-1-APDownload protocol
IHC protocol for MAP7 antibody 13446-1-APDownload protocol
IF protocol for MAP7 antibody 13446-1-APDownload protocol
IP protocol for MAP7 antibody 13446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

BMC Biol

Two-step interphase microtubule disassembly aids spindle morphogenesis.

Authors - Nunu Mchedlishvili
humanIF

Mol Biol Cell

Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.

Authors - Erin M Masucci
humanWB,IHC

Adv Sci (Weinh)

N6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation

Authors - Yin Zhao