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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP7D1 Polyclonal antibody (20788-1-AP)

    WB analysis using 20788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20788-1-AP (MAP7D1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MAP7D1 Polyclonal antibody (20788-1-AP)

    IP experiment of HEK-293 using 20788-1-AP

    IP result of anti-MAP7D1 (IP:20788-1-AP, 4ug; Detection:20788-1-AP 1:30000) with HEK-293 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MAP7D1 Polyclonal antibody (20788-1-AP)

    IHC staining of mouse heart using 20788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MAP7D1 Polyclonal antibody (20788-1-AP)

    IHC staining of mouse skeletal muscle using 20788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MAP7D1 Polyclonal antibody (20788-1-AP)

    IF Staining of U-251 using 20788-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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MAP7D1 Polyclonal antibody

MAP7D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20788-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Proline/arginine-rich coiled-coil domain-containing protein 1, PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MDA-MB-231 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20788-1-AP targets MAP7D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP7D1 fusion protein Ag14385 相同性解析による交差性が予測される生物種
Full Name MAP7 domain containing 1
Calculated molecular weight 841 aa, 93 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC003083
Gene Symbol MAP7D1
Gene ID (NCBI) 55700
RRIDAB_3669347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KQU3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).

Protocols

Product Specific Protocols
WB protocol for MAP7D1 antibody 20788-1-APDownload protocol
IHC protocol for MAP7D1 antibody 20788-1-APDownload protocol
IF protocol for MAP7D1 antibody 20788-1-APDownload protocol
IP protocol for MAP7D1 antibody 20788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols