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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, canine and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28007-1-AP

Synonyms

c Jun N terminal kinase 3, FLJ12099, FLJ33785, JNK, JNK1, JNK1/2/3, JNK2, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, MAPK8, MAPK8/9/10, MAPK9, p493F12, p54bSAPK, PRKM10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JNK Polyclonal antibody (28007-1-AP)

    WB analysis using 28007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using JNK Polyclonal antibody (28007-1-AP)

    IHC staining of mouse brain using 28007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using JNK Polyclonal antibody (28007-1-AP)

    IHC staining of mouse brain using 28007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using JNK Polyclonal antibody (28007-1-AP)

    IHC staining of mouse cerebellum using 28007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28007-1-AP (MAPK8/9/10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using JNK Polyclonal antibody (28007-1-AP)

    IHC staining of mouse cerebellum using 28007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28007-1-AP (MAPK8/9/10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNeuro-2a cells, MDCK cells, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28007-1-AP targets JNK in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, canine samples.

Tested Reactivity Human, Mouse, canine
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK fusion protein Ag27823 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 10
Calculated molecular weight 464 aa, 53 kDa
Observed molecular weight 45-48 kDa, 54-57 kDa
GenBank accession numberBC065516
Gene symbol JNK3
Gene ID (NCBI) 5602
RRIDAB_2881035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JNK, also named MAPK10 (mitogen-activated protein kinase 10), belongs to MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is mainly expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. JNK has 3 isoforms of 48-53 kDa.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 28007-1-APDownload protocol
IHC protocol for JNK antibody 28007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

VSIG4 inhibits RANKL-induced osteoclastogenesis by enhancing Nrf2-dependent antioxidant response against reactive oxygen species production

Authors - Jiansen Miao
View Article