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  • KD/KO Validated

MAPK12 Polyclonal antibody

MAPK12 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20184-1-AP

Synonyms

ERK 6, ERK3, ERK6, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, MAPK12, P38GAMMA, PRKM12, SAPK 3, SAPK3


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Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20184-1-AP is MAPK12 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPK12 fusion protein Ag14073 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 12
Calculated molecular weight 367 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC015741
Gene symbol MAPK12
Gene ID (NCBI) 6300
RRIDAB_10665949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAPK12 antibody 20184-1-APDownload protocol
IHC protocol for MAPK12 antibody 20184-1-APDownload protocol
IP protocol for MAPK12 antibody 20184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

Authors - Shutao Zheng
  • KD Validated
ratWB

BMC Endocr Disord

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.

Authors - Shuman Wang
humanIHC

Clin Transl Oncol

A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma

Authors - Yue Liu