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KD/KO Validated

MAPK12 Polyclonal antibody

Name: MAPK12 Polyclonal antibody
Brand: Proteintech
SKU: 20184-1-AP

MAPK12 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20184-1-AP

Synonyms

ERK 6, ERK3, ERK6, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, MAPK12, P38GAMMA, PRKM12, SAPK 3, SAPK3

Validation Data Gallery

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AllWBIPIHC

WB analysis using 20184-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 20184-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 20184-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 20184-1-AP
IP result of anti-MAPK12 (IP:20184-1-AP, 4ug; Detection:20184-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.
IHC staining of mouse heart using 20184-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 20184-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 20184-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 20184-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 20184-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	mouse heart tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 3 publications below

Product Information

The immunogen of 20184-1-AP is MAPK12 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAPK12 fusion protein Ag14073 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase 12
Calculated molecular weight	367 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC015741
Gene symbol	MAPK12
Gene ID (NCBI)	6300
RRID	AB_10665949
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MAPK12 antibody 20184-1-AP	Download protocol
IHC protocol for MAPK12 antibody 20184-1-AP	Download protocol
IP protocol for MAPK12 antibody 20184-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Tumour Biol Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma. Authors - Shutao Zheng KD Validated View Article
rat	WB	BMC Endocr Disord The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas. Authors - Shuman Wang View Article
human	IHC	Clin Transl Oncol A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma Authors - Yue Liu View Article
human,mouse	WB,IHC	J Orthop Translat Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis Authors - Nana Geng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAPK12 Polyclonal antibody

MAPK12 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 20184-1-AP

WB analysis of human heart using 20184-1-AP

WB analysis of mouse skeletal muscle using 20184-1-AP

IP experiment of mouse skeletal muscle using 20184-1-AP

IHC staining of mouse heart using 20184-1-AP

IHC staining of mouse heart using 20184-1-AP

IHC staining of human heart using 20184-1-AP

IHC staining of human heart using 20184-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.

A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma

Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis