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KD/KO Validated

SAPK4 Polyclonal antibody

Name: SAPK4 Polyclonal antibody
Brand: Proteintech
SKU: 10217-1-AP

SAPK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10217-1-AP

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

Validation Data Gallery

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AllWBIHC

WB analysis using 10217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10217-1-AP (SAPK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 10217-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10217-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10217-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10217-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10217-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10217-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10217-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	U-937 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected in	human pancreas cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below

Product Information

10217-1-AP targets SAPK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	hamster, human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SAPK4 fusion protein Ag0255 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase 13
Calculated molecular weight	38 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC000433
Gene symbol	MAPK13
Gene ID (NCBI)	5603
RRID	AB_2877727
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SAPK4 antibody 10217-1-AP	Download protocol
IHC protocol for SAPK4 antibody 10217-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
hamster	WB	Sci Rep Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome. Authors - Lingling Han View Article
human	WB,IHC	Tumour Biol Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma. Authors - Shutao Zheng KD Validated View Article
rat	WB	BMC Endocr Disord The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas. Authors - Shuman Wang View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAPK4 Polyclonal antibody

SAPK4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 10217-1-AP

IHC staining of human pancreas cancer using 10217-1-AP

IHC staining of human pancreas cancer using 10217-1-AP

IHC staining of human liver cancer using 10217-1-AP

IHC staining of human liver cancer using 10217-1-AP

IHC staining of human liver cancer using 10217-1-AP

IHC staining of human liver cancer using 10217-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.