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  • Featured Product
  • KD/KO Validated

MAPK13 Monoclonal antibody

MAPK13 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B12A6

Cat no : 60252-1-Ig

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of Ramos using 60252-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of HepG2 using 60252-1-Ig

    WB result of MAPK13 antibody (60252-1-Ig, 1:2000) with si-Control and si-MAPK13 transfected HepG2 cells.

  • Western Blot (WB) analysis of Daudi cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of Daudi using 60252-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of HEK-293 using 60252-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAPK13 Monoclonal antibody (60252-1-Ig)

    IHC staining of human pancreas cancer using 60252-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAPK13 Monoclonal antibody (60252-1-Ig)

    IHC staining of human pancreas cancer using 60252-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRamos cells, HepG2 cells, Daudi cells, HEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60252-1-Ig is MAPK13 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAPK13 fusion protein Ag21240 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 13
Calculated molecular weight 38 kDa
Observed molecular weight38-42 kDa
GenBank accession numberBC000433
Gene symbol MAPK13
Gene ID (NCBI) 5603
RRIDAB_2881373
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK13(Mitogen-activated protein kinase 13) is also named as PRKM13, SAPK4 and belongs to the protein kinase superfamily. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.

Protocols

Product Specific Protocols
WB protocol for MAPK13 antibody 60252-1-IgDownload protocol
IHC protocol for MAPK13 antibody 60252-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols