MAPK15 Polyclonal antibody

MAPK15 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13452-1-AP

Synonyms

ERK 7, ERK 8, ERK7, ERK8, MAP kinase 15, MAPK 15, MAPK15


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Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13452-1-AP targets MAPK15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPK15 fusion protein Ag4255 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 15
Calculated molecular weight 544 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC028034
Gene symbol MAPK15
Gene ID (NCBI) 225689
RRIDAB_2877950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAPK15 antibody 13452-1-APDownload protocol
IHC protocol for MAPK15 antibody 13452-1-APDownload protocol
IP protocol for MAPK15 antibody 13452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Metastasis-associated gene MAPK15 promotes the migration and invasion of osteosarcoma cells via the c-Jun/MMPs pathway.

Authors - Zexin Su